Flow cytometry principle with main focus on practical data analysis by flowjo software

 

Talking about flow cytometry technique, various topics may cross the mind of a researcher: FACS technique, cell viability, stem cell and cancer cell phototype, autoimmune diseases, diagnosis and follow-up of leukemias, etc. In general. Flow cytometry, as one of the fascinating techniques of research and clinical laboratories, can be more practical and useful than we think. Using this technique, it is possible to study the surface and intracellular markers (examining everything that happens in and out of the cell). The good news is that using this technique, it is possible to study different types of eukaryotic and prokaryotic cells. As a result, fortunately the target audience of this workshop includes all the different trends in the field of biology.
Definitely to interpret its data, we need to learn software related to raw data analysis. In this workshop, we intend to first talk about the principles and basis of flow cytometry technique and of course its various applications, then in the practical part we get acquainted with flowjo software, its statistical analysis and analysis of its charts (Gating topics, histograms, quadrants). The main focus of this workshop is specifically on cell cycle studies, apoptosis (Annexin-PI) and immunophenotyping.

 

Finally, all participating members will be able to design projects based on various tests in the field of flow cytometry technique and analyze the resulting data statistically and graphically.

 

Instructor:

Leila Khani, MSc in immunology (Flow cytometry department of Nobel Lab)

 

Contents:

• The principle of flow cytometry
• Antibody panel design, concept of compensation and its importance on data analysis, FMO control
• Theoretical and practical principles of immunophenotyping, cell cycle and apoptosis (Annexin-PI assay)
• Evaluation of prokaryotic viability using flow cytometry evaluation

 

Case Study Contents:

• Flowjo software (data & statistical analysis) & chart
• Analysis of the data of cell cycle, apoptosis and immunophenotyping

6-7 October 2020 (15-16 Mehr 1399)

8:30-10:30 / 11:30-13:30

Registration Fee: 2,500,000 IRR

(Discount is available for students and group registration):

BSc and MSc:2.250.000 IRR

Group registration(3 persons and more): 2.000.000 IRR

Nobel Lab rewards 50% refund for the top 2 qualified participants.

Qualified participants receive signed certificate authorized by University of Isfahan and OMITREEN.

Audience group: Students (BSc, MSc, or PHD) of biology, medicine, medical engineering and computer sciences clinical laboratory technician.